The LipidWeb blank

Sphingolipids - Chemistry and Biochemistry
An Introduction



1.  Sphingolipid Basics

The sphingolipids comprise a wide range of complex lipids in which the defining component is a long-chain or sphingoid base that may be linked to a fatty acid via an amide bond. The root term “sphingo-” was first coined by J.L.W. Thudichum in 1884 because the enigmatic nature of the molecules reminded him of the riddle of the sphinx. The term “sphingolipide” was introduced by Herbert Carter and colleagues in 1947. While they are perhaps less enigmatic than they once were, sphingolipids are extremely versatile molecules that continue to fascinate as new knowledge is gained of their functions in healthy and diseased animal and plant tissues. They are found in only a few bacterial genera but especially Sphingomonas and Sphingobacterium. Novel structures are reported frequently, and as an example at the last count, 188 of the complex sphingolipids classified as gangliosides with variations in the carbohydrate chain had been characterized in vertebrates alone.

Typical sphingolipids

A long-chain base, such as sphingosine, is the basic element and is the simplest possible functional sphingolipid, but ceramides, which contain a fatty acid linked by an amide bond to this, are the precursors of a multitude of phospholipids and glycolipids with an immense range of functions in tissues. In addition, long-chain bases and ceramides have important biological properties in their own right, for example in relation to intra- and inter-cellular molecular signalling, especially in animal cells, while another relatively simple sphingolipid, sphingosine-1-phosphate, is now recognized as a key factor in countless aspects of animal metabolism. The properties and functions of the complex sphingolipids are quite distinct from the properties of those of the glycerophospho- and glycolipids. For example, sphingomyelin has structural similarities to phosphatidylcholine, but has very different physical and biological properties, while the complex oligoglycosylceramides and gangliosides have no true parallels amongst the glycerolipids. There are appreciable differences in sphingolipid compositions and metabolism between animal and plant cells, although there are also some important similarities.

Complex sphingolipids are located mainly in the plasma membrane of mammalian cells where they have a structural function and also serve as adhesion sites for proteins from the extracellular tissue. However, they have analogous intracellular functions in all cellular compartments, including the nucleus. Although less is known of the role they play in plants, it has become apparent that complex sphingolipids are much more abundant in plant membranes than has been realized. A distinctive property of sphingolipids in membranes is that they spontaneously form microdomains termed 'rafts', usually in conjunction with cholesterol, where specific proteins such as enzymes and receptors congregate. Thus, in addition to their direct effects on metabolism, sphingolipids affect innumerable aspects of biochemistry indirectly via their physical properties. This is such an important topic that it now has its own web page here.

While it may be obvious that a well-balanced sphingolipid metabolism is important for health, increasing evidence has been acquired to demonstrate that impaired sphingolipid metabolism and function are involved in the patho-physiology of many of the more common human diseases. These include diabetes, various cancers, microbial infections, Alzheimer's disease and other neurological syndromes, and diseases of the cardiovascular and respiratory systems. Sphingolipids and their metabolism are therefore likely to prove of ever increasing interest to scientists.


2.  General Comments on Sphingolipid Metabolism

The biosynthesis and catabolism of sphingolipids involves a large number of intermediate metabolites, all of which have distinctive biological activities of their own. In animals, the relationships between these metabolites have been rationalized in terms of a ‘sphingomyelin or ceramide cycle’.

The sphingomyelin cycle

Many different enzymes (and their isoforms) are involved, and their activities depend on a number of factors, including intracellular locations and mechanisms of activation. Each of the various compounds in these pathways has characteristic metabolic properties, and these are discussed in more detail on the web pages describing the individual sphingolipids. Thus, free sphingosine and other long-chain bases, which are the primary precursors of ceramides and thence of all the complex sphingolipids, function as mediators of many cellular events, for example by inhibiting the important enzyme protein kinase C. Ceramides are involved in cellular signalling, and especially in the regulation of apoptosis, and cell differentiation, transformation and proliferation, and most stress conditions. In contrast, sphingosine-1-phosphate and ceramide-1-phosphate promote cellular division (mitosis) as opposed to apoptosis, so that the balance between these lipids and ceramide and/or sphingosine levels in cells is critical.

Similarly, the ‘structural’ sphingolipids, such as sphingomyelin, monoglycosylceramides, oligoglycosylceramides, gangliosides and sulfatides, all have unique and characteristic biological functions, a high proportion of which are due to their physical properties and location within raft domains of membranes. Most of the reactions in the sphingomyelin cycle are reversible and the relevant enzymes are located in the endoplasmic reticulum, Golgi, plasma membrane and mitochondria, but the more complex sphingolipids are digested in the lysosomes.

Metabolic pathways that are comparable to those of the sphingomyelin cycle are believed to occur in plants, although they have not been studied as extensively as those in animals (sphingomyelin itself does not occur in plants). However, sphingolipid metabolites such as sphingosine-1-phosphate (or analogues) have been linked to programmed cell death, signal transduction, membrane stability, host-pathogen interactions and stress responses, for example.

Sphingolipid biosynthetic pathways in plants

Plants also have a unique range of complex sphingolipids in their membranes, such as ceramide phosphorylinositol and the phytoglycosphingolipids, and these are now known to constitute a higher proportion of the total lipids than had hitherto been supposed, although their functions have hardly been explored.


3.  Fatty acid Components of Sphingolipids

The fatty acids of sphingolipids are very different from those of glycerolipids, consisting of very-long-chain (up to C26) odd- and even-numbered saturated or monoenoic and related 2-R-hydroxy components, while even longer fatty acids (C28 to C36) occur in spermatozoa and the epidermis. In plants, 2-hydroxy acids predominate sometimes accompanied by small amounts of 2,3-dihydroxy acids. Polyunsaturated fatty acids are only rarely present, although sphingomyelins of testes and spermatozoa are exceptions in that they contain such fatty acids, which are even longer in chain-length (up to 34 carbon atoms) and include 28:4(n-6) and 30:5(n-6). Skin ceramides also contain unusual very-long-chain fatty acids, while yeast sphingolipids are distinctive in containing mainly C26 fatty acids.

Very-long-chain saturated and monoenoic fatty acids are produced by elongases (ELOVL) in the endoplasmic reticulum of cells, and there is increasing evidence that specific isoforms are involved in the biosynthesis of certain ceramides. For example, ELOVL1 has been linked to the production of ceramides with C24 fatty acids (see our web page on long-chain bases). Hydroxylation is effected by a fatty acid 2-hydroxylase in mammals, i.e. an NAD(P)H-dependent monooxygenase, which is an integral membrane protein of the endoplasmic reticulum. It converts unesterified long-chain fatty acids to 2‑hydroxy acids in vitro and probably also in vivo. For example, experimental evidence has been obtained that is consistent with 2‑hydroxylation occurring at the fatty acid level prior to incorporation into ceramides in the brain of mice. Similarly, in skin, 2‑hydroxy and non-hydroxy fatty acids as their CoA esters are used with equal facility for ceramide biosynthesis by ceramide synthases. It is possible that a proportion of the odd-chain fatty acids in brain are synthesised by peroxisomal α-oxidation of the 2‑hydroxy acids. In plants, it appears that 2‑hydroxyl groups are inserted into fatty acyl chains while they are linked to ceramide, as ceramide synthase does not accept hydroxy fatty acids in vitro at least. Two fatty acid 2‑hydroxylases have been found in Arabidopsis, with one specific for very-long-chain fatty acids and one for palmitic acid.

Although the fatty acids are only occasionally considered in terms of the biological functions of sphingolipids, their influence is considerable, especially but not only in relation to their physical properties and function in membranes. For example, synthetic glycerolipids must contain very-long-chain fatty acids (C26) to allow growth in yeast mutants lacking sphingolipids, probably by stabilizing the proton-pumping enzyme H+-ATPase. Similarly, ceramides containing different fatty acids can be used in highly specific ways. For example, in fungi, C16 or C18 hydroxy acids are used exclusively for synthesis of glucosylceramide, while those containing very-long-chain C24 and C26 hydroxy acids are used only for synthesis of glycosyl inositol phosphorylceramide anchors for proteins. In plants, sphingolipids containing 2-hydroxy acids are protective against oxidative stress.


4.  Links between Glycerolipid and Sphingolipid Metabolism

Sphingolipid metabolism and glycerolipid metabolism have been widely treated as separate sciences until relatively recently, partly for historical reasons and partly because the analysis of the two lipid groups required different approaches and skills. However, there are many areas where the two overlap, not least because phosphatidylcholine is the biosynthetic precursor of sphingomyelin in animal cells, while in plants and fungi, phosphatidylinositol is the biosynthetic precursor of ceramide phosphorylinositol. In contrast, ethanolamine phosphate derived from the catabolism of sphingolipids via sphingosine 1-phosphate is recycled for the biosynthesis of phosphatidylethanolamine.

In addition, there are several examples of phosphoinositides and other complex lipids binding to enzymes of sphingolipid metabolism, either as part of a regulatory function that controls their activity or to facilitate their location to various membranes. Thus, sphingosine kinase 2, one of the enzymes responsible for the biosynthesis of sphingosine 1-phosphate, binds to phosphatidylinositol monophosphates, while the ceramide kinase responsible for the biosynthesis of ceramide 1-phosphate requires phosphatidylinositol 4,5-bisphosphate to function. Similarly, the CERT protein involved in ceramide transport has a binding site for phosphatidylinositol 4-phosphate. Again, the interactions are not solely in one direction as ceramide 1-phosphate (with phosphatidylinositol 4,5-bisphosphate) binds to the specific phospholipase A2 (cPLA2α) responsible for the hydrolysis of phosphatidylinositol and thence the release arachidonic acid for eicosanoid production. Other than the phosphoinositides, phosphatidylserine activates the neutral sphingomyelinase in brain.

The glyceroglycolipid seminolipid from male reproductive tissues and the sphingolipid sulfates have structural elements in common, and they use the same enzymes to introduce the carbohydrate and then the sulfate group to diacylglycerol and ceramide intermediates, respectively.


Suggested Reading



Lipid listings Credits/disclaimer Updated: May 3rd, 2017 Author: William W. Christie LipidWeb icon