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Mass Spectrometry of 4,4-Dimethyloxazoline
(DMOX) Derivatives of Fatty Acids



4,4-Dimethyloxazoline (DMOX) derivatives give excellent mass spectra of fatty acids with electron-impact ionization that often permit unequivocal identification. The gas chromatographic properties of DMOX derivatives are better than those of other nitrogen-containing derivatives, and many analysts consider them the best single derivative available for identification of fatty acids. However, when there are functional groups near the terminal end of the molecule, problems arise. One further drawback is that DMOX derivatives are not very stable chemically, as ring opening occurs rapidly on storage in the presence of traces of moisture (see our web page on preparation of derivatives). However, if analysts are aware of potential pitfalls, these difficulties can be overcome with assistance from the documents in this section of the website. Some of the following web documents also discuss the spectra of analogous pyrrolidide derivatives because of similarities in the fragmentation patterns. These web pages are updated regularly as new information and mass spectra become available.


Recommended Book: Christie, W.W. and Han, X. Lipid Analysis - Isolation, Separation, Identification and Lipidomic Analysis (17th edition), 446 pages (Oily Press, Woodhead Publishing and now Elsevier) (2010) - at Science Direct.

Credits/disclaimer Updated: August 29th, 2018 Author: William W. Christie LipidWeb icon