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Bibliography of Mass Spectrometry of Fatty Acids

Mild Ionization Techniques

Scottish thistleAtmospheric-pressure chemical-ionization (APCI)and electrospray mass spectrometry are the mildest ionization techniques available, but they are particularly effective linked to high-performance liquid chromatography, as it can tolerate relatively high solvent flow rates. In conjunction with tandem-MS techiques, it can give useful structural information on fatty acids. Another very promising technique involves acetonitrile-chemical-reaction tandem mass spectrometry in the gas phase for locating double bonds in fatty acid methyl esters (from the laboratory of Professor J.T. Brenna mainly). In the mass spectrometer, an ion (m/z = 54), formed by reaction of acetonitrile with itself, adds across the double bond in a cycloaddition reaction. When this nascent complex undergoes collision-induced dissociation, two diagnostic ions emerge. One of these results from loss of the hydrocarbon end of the FAME, and the other ion from loss of the methyl ester end, and together, these locate the positions of the double bonds in the FAME. Not only that, but they can determine the cis/trans geometry of the double bonds.

The following references were collected as part of our regular literature reviews for our own research purposes. The literature coverage here will be far from complete. References are listed alphabetically by the first author of a paper, but not necessarily chronologically by that author. New references may be added at irregular intervals - check the bottom of the page for when it was last updated.

Credits/disclaimer Updated: December 21st, 2018 Author: William W. Christie LipidWeb icon